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Dear IVF.net subscriber,
Welcome to the latest issue of IVF News.
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<< Add your questions here >>
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Title |
Date Added |
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Replies |
| 1 |
Stability of pH during media equilibration
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12 January 2010 |
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1
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| Dear All, I would like to know if anybody has monitored the stability of medium pH during equilibration ( in our laboratory we use G1 v5 PLUS, Vitrolife and pH Meter Version 2 from RI). We have done 4 24-hours measurements:
1) in two cases after 8 hours of equilibration pH-meter showed the minimal value and this reading was stable for next 8 hours. Then pH increased at about 0,03-0,04 in the last 8-hours of measurement.
2) in the next two cases after 8 hours of equilibration pH-meter showed the minimal value but this reading was stable only for 2-3 hours and then the pH increased at about 0,03-0,04 too.
Has anybody noticed a similar tendency ?
Zofia |
Replies
Re: Stability of pH during media equilibration
The observation is appreciable, which pH meter are you using, and what was the volume and container of the media? Did you measured the pH while inside the co2 incubator and what was the set co2 concentration (%), was that a triple gas or a single gas incubation. please detail the procedure of measurement.
I hope pH control for IVF cultures is worth a long discussion. I will request the experts for the same.
ALOK Teotia
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