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In Vitro Fertilization: A Practical Approach

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Dear IVF.net subscriber,
Welcome to the latest issue of IVF News.
The ASRM in Atlanta starts this weekend. If you would like to report on what you feel is important news from the conference or would like to share your photos you can do so here.
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IVF Jobs
<< Add your jobs here >>
| # |
Position |
Closing Date |
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| 1 |
Medical Technologist - Andrology
Cleveland Clinic Main Campus Andrology Department
Cleveland , United States
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| 2 |
Embryologist
CARE fertility
Northampton, United Kingdom
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16 November 2009 |
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| 3 |
Trainee Embryologist/Andrologist
Southampton University Hospitals NHS Trust
Southampton, United Kingdom
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30th October 2009 |
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| 4 |
Embryologist
Edinburgh IVF Centre c/o Spire Murrayfield Hospital
Edinburgh, United Kingdom
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Until position filled |
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| 5 |
Gynecologist/IVF consultant
Moe
Dubai, United Arab Emirates
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Until the position is filled |
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| 6 |
Experienced Senior/Clinical Andrologist
CRGH
London, United Kingdom
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Until position is filled |
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| 7 |
Fertility Nurse
Bourn Hall Clinic
Cambridge, United Kingdom
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31st October 2009 |
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| 8 |
IVF Nurse Manager
The London Women's Clinic
London, United Kingdom
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25/10/2009 |
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| 9 |
Experienced Embryologist
Fertility PLUS
Auckland, New Zealand
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When filled |
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| 10 |
Senior Fertility Nurse/Sister
Benenden Hospital Trust
Cranbrook, Kent, United Kingdom
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When position is filled |
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IVFMail
<< Add your questions here >>
| # |
Title |
Date Added |
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Replies |
| 1 |
Optimum CO2 level
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14 October 2009 |
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1
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| I have been using medicult ivf medium for culturing embryos, and I had to raise the CO2 level to 6.7% to achieve good grade embryos, despite that the company suggests to use 5% CO2 only. i now attend to use Cook medium instead of Medicult, how do i know which CO2 level is best with Cook?? It recommends 6% ! but is that enough to rely on ?? my Lab is at the same level of the sea! do i have to use a ph meter to know what CO2 level is best??
NJ |
| 2 |
Hands on Training
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13 October 2009 |
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0
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| Which Centre in UK (or Europe)would be the best centre to visit to fine tune our skills.
SPG |
| 3 |
Salary for embryologist in Qatar??
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12 October 2009 |
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0
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| Can somebody please let me know about the salary for an Embryologist in Qatar. Thank you
Suran Rajapakse |
Replies
Re: Optimum CO2 level
Optimum CO2 Back to the basics - how are you measuring your CO2, and what is the pH of the media, with and without protein supplementation at 6.7% CO2? Are you targeting a specific pH range, or just using the embyrology to determine CO2%?
And if you are happy with your embryology and clinical pregnancies under these conditions, you could target the same pH with Cook media to start with, regardless of what the company states. I would definitely do pH trials before placing any embryos into the Cook media.
Also ask Cook how they determined the recommended pH - were they just targeting a certain pH, and do they have clinical data to back it up - and what altitude and laboratory conditions did they encounter while determining their pH values?
Within patient comparisons, splitting the embryos up for each patient into the two separate media, could be useful as well - as long as the pH of both media are similar. Donor egg cycles are a good place to start for this.
Good luck!
Mike
Michael L. Reed Center for Reproductive Medicine of New Mexico
Re: Seeding embryos
Planer Freezer The number of straws you would put in the goblet has a lot of variables, for example the size straws being used, how tightly packed the straws are etc and has an effect on the airflow and temperature accuracy of the straw.
We suggest to use the 3 or 4 place straw holders which are provided with the freezer because there is better circulation of cooling around the sample ensuring absolute accuracy of temperature. These straw holders are also available to order should you have misplaced them or require any more.
Regarding seeding there is some interesting information if you search ivf.net. Here is one I found which may be of interest to you:
http://www.ivf.net/ivf/poor_embryo_survival_after_thaw-o2042.html
Ravi Dattani
Re: Equilibrate hepes media
ORIGINAL PACKINGS are gas tight so they (hepes media) can be equilibrated inside the co2 incubators provided the cap is not loosened, however at times there could be wastage. alternatively one can use glass containers with appropriately fit silicon stoppers.
Alok Teotia MAX HEALTHCARE
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