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Dear IVF.net subscriber,
Welcome to the latest issue of IVF News.
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IVFMail
<< Add your questions here >>
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Title |
Date Added |
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Replies |
| 1 |
Embryology training programs
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02 October 2009 |
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0
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| Hi. I am looking for institute or clinic which offer short/long term training programs in embryology/ART techniques in USA or UK. I would really appreciate your help. Kind Regards Evangelia
Evangelia Siambouri |
| 2 |
Culture Dishes
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01 October 2009 |
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1
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| We have recurring failure growing frozen mouse embryos to the blastocyct stage. The mouse embryos are grown in human embryo media as a QA/QC procedure. The mouse embryos develop to the morula stage and then they blocked and degenerate. Photos of the affected mouse embryos show a large void between the embryo and the zona. The cytoplasm is very fragmented and disorganized. They have confirmed that their protein passed their previous QC checks. Several different sources of protein were used...
Antonia Gilligan |
| 3 |
Failure to fertilize in a few cases
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01 October 2009 |
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0
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| I have recently experienced a failure to fertilize in a few cases in a clinical IVF lab. In these cases, the eggs have been processed for ICSI. 16 hrs following injection there was no consistent sign of fertilization. In some cases, no pronuclei were observed but the second polar body was present. At 24 hrs there faint structures appeared but fertilization was questionable. At 40 hrs pronuclei were present in all oocytes but instead there were multiple nuclei ranging from 3 or more. ..
Antonia Gilligan |
| 4 |
equilibrate hepes media
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30 September 2009 |
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1
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| Is it OK to warm Hepes media in a co2 incubator as long as the cap is tight?
janis |
| 5 |
Best Choice for GnRH Down Regulation Protocol.
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28 September 2009 |
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0
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| Hi, I have a patent of 36 years old. She is having secondary infertility for the past 12 years. She conceived spontaneously only once in 1998 and later on she became infertile. She is having regular menstural cycle. Her BMI is around 27. She had 8 cycles of ovulation induction with CC. and 4 cycles with CC and HMG combined. She had laparoscopy/laparotomy in which it was found the she has tubal adhesions. So apparently she is having infertility due to tubal factor. Which down regulation protocol ..
Rino |
Replies
Re: Culture Dishes
Mouse embyros failing in Nunc
Hi Antonia. A good question - Dean Morbeck with Mayo has an abstract for the upcoming ASRM meeting about culturing mouse embryos either alone or in groups for QC purposes. These embryos were commercial in origin, one-cell I believe, and the sensitivity of the embryos grown individually was much greater, to toxins, than embryos grown in groups. Dean was using well dishes for his study.
How were the embryos grown in your scenario with the Nunc dishes?
Were they grown individually or in groups? I'm also assuming oil overlay.
The GPS dish allows for individual growth tracking and embryo cross-talking at the same time, so perhaps this is part of the solution to your question, if the embryos were grown individually. If not...
I did some titration work with the embryo toxicity testing medium from the AAB - and I was able to reduce the amount of toxin to levels that stopped the formation of the blastocyst - just as you described. A low level toxin leached perhaps from the dishes? The toxin in question, from the toxicity challenge, was, I believe, formaldehyde. Patrick Quinn could tell you for certain, and this was probably 6 or more years ago. I'll did around for the data.
I was also able to demonstrate movement of the toxin from wells without mineral oil, into the atmosphere, and into wells that did not have toxin, and that were also not covered by mineral oil. A four well dish with the cover in place. Very cool.
I'd love to hear more. Best wishes.
Mike
Michael L. Reed Center for Reproductive Medicine of New Mexico
Re: Equilibrate hepes media
Try it - you could use a simple color indicator 'control' tube to compare to your equilibrated container to see if your container is gas tight. A lot of containers are not. If your media has phenol red, your media will be yellow if your container allows any of the CO2 to leak in.
I've tried it - I've had Hepes media in tightly capped tubes turn yellow - and in snap top tubes - so be cautious.
Better to use a warming block, or other similar device, just to be sure.
Mike
Michael L. Reed Center for Reproductive Medicine of New Mexico
Re: Minc v/s Astec incubator
Incubators
You should also consider the new incubator from Planer in the UK www.planer.com - It has the same design as the minc and also includes built in battery back-up. I have used one for a limited number of research embryos and it appeared to perform well (blastocyst development from poor grade embryos). Email the guys at planer for a price as there doesn't seem to be one on their website
Paul Knaggs
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Thank You |
This announcement is made possible
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Reports and licence committee minutes relating to serious incidents in fertility clinics are to be published for the first time, the Human Fertilisation and Embryology Authority has announced.
Donor conceived people and donors will have new rights to access information about themselves held on the HFEA register from Thursday 1 October.
The number of women undergoing IVF treatment has risen once again, according to the latest figures released by the fertility regulator, the Human Fertility and Embryology Authority (HFEA) today.
The single greatest change to affect the UK fertility sector in nearly two decades will take place tomorrow, Thursday 1 October, as the new Human Fertilisation and Embryology Act 1990 (as amended) comes into force.
Bourn Hall Clinic
