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In Vitro Fertilization: A Practical Approach

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Dear IVF.net subscriber,
Welcome to the latest issue of IVF News.
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IVF Jobs
<< Add your jobs here >>
| # |
Position |
Closing Date |
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| 1 |
Fertility/Gynaecology Nurse 
Create Health Clinic
West Wimbledon, United Kingdom
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When the position is filled. |
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| 2 |
IVF Specialist/Senior Clinical Fellow in Reproductive Medicine 
Create Health
West Wimbledon, United Kingdom
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When the position is filled. |
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| 3 |
Trainee Clinical Embryologist
Embryology
Exeter, United Kingdom
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14th September 2009 |
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| 4 |
Senior Fertility Nurse/Sister
Benenden Hospital Trust
Cranbrook, Kent, United Kingdom
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2 October 2009 |
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| 5 |
IVF Laboratory Manager
PIVET Medical Centre
Leederville PERTH, Australia
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30 September 2009 |
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| 6 |
Fertility Nurse
Isis Fertility Centre
Colchester, United Kingdom
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30.9.09 |
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| 7 |
Experienced Clinical Embryologist - Short Term or Long Term
Repromed NZ
Auckland, New Zealand
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When filled |
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| 8 |
Experienced Senior/Clinical Embryologists and Trainee Embryologist
London Women's Clinic
London, United Kingdom
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Until position is filled |
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| 9 |
ANDROLOGIST (PART TIME)
Herts and Essex Fertility Centre
Cheshunt, United Kingdom
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When position filled |
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| 10 |
Senior Clinical Embryologist
Benenden Fertility Centre
Benenden , Cranbrook, United Kingdom
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When position is filled |
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| 11 |
Senior Embryologist
ISIS CLINIC
Nicosia, Cyprus
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IVFMail
<< Add your questions here >>
| # |
Title |
Date Added |
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Replies |
| 1 |
Looking to buy used IVF equipments
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06 September 2009 |
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0
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| We are looking for used Narishige micromanipulator, CO2 incubator, laminar flow hood, sperm and embrio freezers, etc. Kindly contact with the specifications of the equipment and the expected price.
Karen |
| 2 |
question and inquiry
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02 September 2009 |
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1
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| Hi , my name is Talha , currently working as Embryologist in a pharma , I want to ask that how will be sperms counted if there are 40 -50 million sperms phpf i mean will the sperms be in thousands expressed, i.e what wil be count actually if the count is mentioned bove , i am actually not been able to diffrentite 40-50 million per ml and phpf how wil I count it . The other one is regarding Halo Sperm kit , can some body give me technical information , about it ..
talha |
| 3 |
embryo corral dishes
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31 August 2009 |
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0
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| does anyone have experience with the embryo corral dishes?
janis moessner |
Replies
Re: Question and inquiry
A few comments on sperm counting using the sperm per hpf method. From the ESHRE Monographs Manual on Basic Semen Analysis, June 2002, in the section discussing sperm concentration, the text states that 4 sperm per high powered field corresponds roughly to a concentration of 1 m/ml. So if you are counting 40 to 50 sperm phf, then you could assume that the concentration would be approximately 10 to 13 m/ml. This is also assuming that you are using 400X magnification, given that your field of vision is 500 micrometers in diameter, and given that you are using 6 microliter volume of well mixed semen under an 18x18 coverslip on a glass slide. The text states also that older microscopes may have different diameter fields of vision. So, there is a lot of room for error using this method. Yes, this method is likely ok for qualitative semen analysis - a screening method perhaps. But I would consider an alternate method for more accuracy and precision.
The second part to your question regarding the Halosperm kit, this test allows the user to perform sperm decondensation testing, reflecting sperm DNA fragmentation, in-house, using either brightfield microscopy or flurorescent microscopy. The test is fairly easy to perform, with very good step-by-step instructions. Reading the slides is like reading a version of a stained morphology, so you can expect to have a learning curve, with refinement of inter- and intra-technician variation over time. As more programs use the test, and compare the results to clinical outcomes, and assuming we can share information between all of us, then the true utility of this assay can be determined. Like any clinical test, you\'ll have to decide how best to use the data in your setting.
Mike
Michael L Reed Center for Reproductive Medicine of New Mexico
Re: Treatment for spermatocyte I maturation arrest
In-vitro spermatogenesis resumption in men with maturation arrest: relationship with in-vivo blocking stage and serum FSH
Jan Tesarik, Basak Balaban, Aycan Isiklar, Cengiz Alatas, Bülent Urman, Senai Aksoy, Carmen Mendoza and Ermanno Greco
Dr Mohit
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