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In Vitro Fertilization: A Practical Approach

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Dear IVF.net subscriber,
Welcome to the latest issue of IVF News.
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IVF Jobs
<< Add your jobs here >>
| # |
Position |
Closing Date |
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| 1 |
Chief Embryologist/Laboratory Manager 
Create Health
West Wimbledon, United Kingdom
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When the position is filled. |
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| 2 |
Andrologist/Donor Sperm Bank Co-ordinator
London Fertility Centre
London, United Kingdom
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31st July 2009 |
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| 3 |
Fully Trained OR Advanced Trainee
Pedieos IVF Center
Nicosia, Cyprus
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| 4 |
Fertility Nurse
IVF Hammersmith Limited
London, UK
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31 July 2009 |
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| 5 |
IVF Nurse Coordinator
Barbados Fertility Centre
Hastings, Barbados
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July 17 2009 |
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| 6 |
trained/trainee embryologist
Victoria Wing
Woking, United Kingdom
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24th July 2009 |
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| 7 |
Embryologist
The Agora Gynaecology & Fertility Centre
Brighton & Hove, United Kingdom
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when position is filled |
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| 8 |
Embryologist - Maternity Leave Cover
Wessex Fertility Limited
Southampton, United Kingdom
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13th July 2009 |
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| 9 |
Embryologist - Based Anywhere, US or Canada
Cook Medical
Bloomington , United States
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IVFMail
<< Add your questions here >>
| # |
Title |
Date Added |
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Replies |
| 1 |
Vitrification medium
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09 July 2009 |
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0
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| I am hoping to start vitrification for day 3 as well as blastocysts. Can some one recommend me suitable media?
Dr Suran Rajapakse |
| 2 |
CO2 % at high altitudes
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07 July 2009 |
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2
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| Hello! My centre is at a higher altitude around 900 metres above sea level.I need to set the CO2 at 7.8 to 8% to get the Cook media at 7.30 -7.33. I have a senitive,calibrated pH meter (which mesures +/- 0.01) and a digital capnometer calibrated with 5% CO2.This looks quite strange but logical as we need higher co2 % to generate the required PCO2.I need to raise the CO2 by almost 1% to get 0.3 drop in the pH.So I may need to make it more than 8 % if I aim the pH to be around 7.27 - 7...
Dr.Sowmya Dinesh |
Replies
Re: CO2 % at high altitudes
CO2 and altitude Interesting question - altitude does play a role in the partial pressure of CO2 needed to maintain a certain pH, however I think in you case, there might a factor other than altitude contributing to the problem.
My lab is at approximately 6000 feet above sea level, essentially twice the altitude that you have described, 900 meters, and I maintain a pH of around 7.2, using either Global or G media, with approximately 6% CO2. In theory, I would need slightly higher CO2 % than you to maintain the same pH. To the other extreme, we have an IVF colleague in Peru at around 10,000 feet above sea level, and the CO2 % required for similar pH is about 9%.
What protein supplementation are you using? Are your readings with or without protein?
And...what is your local humidity, and do you have CO2 sensors that are dependent upon humidity, rather than infrared? My lab has extremely low humidity, around 6 to 10% on average, and I keep two large pans of water in each incubator. I know that if I let the water levels drop, the sensors do not read correctly. A basic principle, and the incubator manufacturers may not always take that into account with their manuals. You may follow the incubator directions and still not have enough humidity to get proper sensor function.
If that does not solve the problem, then look to other media from a different vendor as a control - most media today are designed to operate at similar pH with either 5 or 6% CO2.
Good luck!
Michael L. Reed Center for Reproductive Medicine of New Mexico
Re: CO2 % at high altitudes
Whatever is the required level of co2 to maintain the pH of the droplet ie. 7.25-7.27. I think my embryos are happy with this pH rather than the level of co2
Dr. RINKU BANERJI Ideal fertilityivf/icsi and genetic centre
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