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Dear IVF.net subscriber,

Welcome to the latest issue of IVF News.

IVF News

IVF news story Embryology Academy for Research & Training will be conducting next IVF training batches in the month of August, 2009.
IVF TRAINING 10 July 2009

IVF news story The HFEA has published its updated Code of Practice for UK clinics. It includes legal changes required under the HFE Act 2008, policy amendments and is published alongside revised and updated consent forms and Directions.
HFEA 07 July 2009 - 0 Comments.


IVF Jobs

<< Add your jobs here >>

# Position Closing Date
1 Chief Embryologist/Laboratory Manager  featured
Create Health
West Wimbledon, United Kingdom
 When the position is filled.
2 Andrologist/Donor Sperm Bank Co-ordinator
London Fertility Centre
London, United Kingdom
 31st July 2009
3 Fully Trained OR Advanced Trainee
Pedieos IVF Center
Nicosia, Cyprus
 
4 Fertility Nurse
IVF Hammersmith Limited
London, UK
 31 July 2009
5 IVF Nurse Coordinator
Barbados Fertility Centre
Hastings, Barbados
 July 17 2009
6 trained/trainee embryologist
Victoria Wing
Woking, United Kingdom
 24th July 2009
7 Embryologist
The Agora Gynaecology & Fertility Centre
Brighton & Hove, United Kingdom
 when position is filled
8 Embryologist - Maternity Leave Cover
Wessex Fertility Limited
Southampton, United Kingdom
 13th July 2009
9 Embryologist - Based Anywhere, US or Canada
Cook Medical
Bloomington , United States
 


Resumes

<< Add your resume/CV here >>

# Title Country Photo
1 Sule Dogan
Embryologist/ Andrologist/ Research assistant
USA
 "Female and 27 years old."
2 Mario Iliceto
Trainee Embryologist/ Andrologist
United Kingdom
 "Working as a trainee embryologist/Andrologist"
3 Jay Hackworth
Embryologist/Medical Technologist
United States -
 "ASCP certified Medical Technologist/fully trained Embryologist seeking full/part-time/per-diem position."
4 Nicola Clark
Trainee embryologist
United Kingdom -
 "To find somewhere willing to train me as a clincal embryologist"
5 Natalya Rozanova
Embryologist
United States
 "Looking for a full time embryologist position with IVF lab or clinic."
6 Yara Pujol Lopez
embryologist
Spain -
 "A challenging position as an intermediate-level clinical embryologist."
7 Andrew Glazier PhD
Laboratory Director / Consultant or Senior Embryologist / Andrologist
United Kingdom -
 "Highly experienced Lab director/consultant level embryologist available for locum work in UK, EU, Worldwide."
8 Jillene Wock
Application of Reproductive Physiology in Research or in a Clinical Atmosphere
United States -
 "To obtain a position that utilizes my skills, experience and education to its full potential, as well as challenges me to learn new techniques in the field of reproductive physiology or any related field."
9 shakir hussain
embryologist
Pakistan -
 "Develop carrier in a esteemed organization,fast growing and progress oriented environment by using skills,knowledge,experiences and intellectual abilities."
10 Jaru Harris
Embryologist
United States

More resumes


IVFMail

<< Add your questions here >>

# Title Date Added Replies
1 Vitrification medium 09 July 2009 0
I am hoping to start vitrification for day 3 as well as blastocysts. Can some one recommend me suitable media?
Dr Suran Rajapakse
2 CO2 % at high altitudes 07 July 2009 2
Hello! My centre is at a higher altitude around 900 metres above sea level.I need to set the CO2 at 7.8 to 8% to get the Cook media at 7.30 -7.33. I have a senitive,calibrated pH meter (which mesures +/- 0.01) and a  digital capnometer calibrated with 5% CO2.This looks quite strange but logical as we need higher co2 % to generate the required PCO2.I need to raise the CO2 by almost 1% to get 0.3 drop in the pH.So I may need to make it more than 8 % if I aim the pH to be around 7.27 - 7...
Dr.Sowmya Dinesh



Replies

Re: CO2 % at high altitudes
CO2 and altitude
Interesting question - altitude does play a role in the partial pressure of CO2 needed to maintain a certain pH, however I think in you case, there might a factor other than altitude contributing to the problem.

My lab is at approximately 6000 feet above sea level, essentially twice the altitude that you have described, 900 meters, and I maintain a pH of around 7.2, using either Global or G media, with approximately 6% CO2. In theory, I would need slightly higher CO2 % than you to maintain the same pH. To the other extreme, we have an IVF colleague in Peru at around 10,000 feet above sea level, and the CO2 % required for similar pH is about 9%.

What protein supplementation are you using? Are your readings with or without protein?

And...what is your local humidity, and do you have CO2 sensors that are dependent upon humidity, rather than infrared? My lab has extremely low humidity, around 6 to 10% on average, and I keep two large pans of water in each incubator. I know that if I let the water levels drop, the sensors do not read correctly. A basic principle, and the incubator manufacturers may not always take that into account with their manuals. You may follow the incubator directions and still not have enough humidity to get proper sensor function.

If that does not solve the problem, then look to other media from a different vendor as a control - most media today are designed to operate at similar pH with either 5 or 6% CO2.

Good luck!
Michael L. Reed Center for Reproductive Medicine of New Mexico


Re: CO2 % at high altitudes
Whatever is the required level of co2 to maintain the pH of the droplet ie. 7.25-7.27. I think my embryos are happy with this pH rather than the level of co2
Dr. RINKU BANERJI Ideal fertilityivf/icsi and genetic centre


 

 



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