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In Vitro Fertilization: A Practical Approach

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Dear IVF.net subscriber,
Welcome to the latest issue of IVF News.
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IVF News
IVF Podcast: Jacques Cohen - Embryo Selection
Dr. Jacques Cohen discusses methods of embryo selection.
[ Visit Website ]
IVF Podcasts
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News: 66 year old women is Britain's oldest mother-to-be
The record for Britain's oldest women to give birth will be broken next
month by 66 year old Elizabeth Munro, from Cambridge. It is thought that Ms
Munro, who is single and a successful business woman, travelled to the Ukraine to become pregnant using donor eggs and IVF treatment.
Sarah Spain
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News: Worse health for IVF twins but no harm in embryo freezing
Two studies have provided an insight into the health implications of using assisted reproductive technologies (ARTs). The first finds that twins conceived by ART are more likely to be born preterm, be of low birthweight and to be 60 per cent more likely to be admitted into neonatal intensive care. The second finds that embryos frozen shortly after cell division had begun are no more likely to lead to premature or underweight birth than fresh embryos.
Benjamin Jones
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Announcement: Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissue and Organs (CRYO)
The 1st International Congress on
Controversies in Cryopreservation of Stem Cells, Reproductive Cells,
Tissue and Organs (CRYO)
Valencia, Spain, April 22-25, 2010
[ Visit Website ]
CRYO Valencia
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IVF Mail
Ask your questions here
Replies
Re: Lab cleaning?
We like to use 70% ETOH for the first cleaning, then a re-cleaning with a quaternary ammonium based cleaners. (0.1-2.0% in water). This is followed by a sterile DI water rinse with a final rinse with 6% H202 in sterile DI water. The last rinse will also disinfect and leave only water and oxygen.
Antonia V. Gilligan Alpha Environmental, Inc.
Re: Lab cleaning?
Sameer,
The best, cheapest and the latest is to clean the incubators with H202. There are other products like Fertspray used in cleaning the incubators.
Do join the ivf forum and you will be able to learn more about cleaning the incubators.
Ather Husain Taiba hospital, Kuwait
Re: Premix
Premix not an option for Heracell Hi. You cant use premixed gas for 100% CO2 incubator like Heracell. You would have to purchase a O2 control technology to be incorporated into the CO2 incubator. Even if you have the O2 control, you can't use premix gas. You have to use CO2 and pure nitrogen gasses to displace the atmospheric oxygen concentration (ie:20% ATM). That's how it works for a low O2 concentration. But I doubt you can purchase an O2 control for your current CO2 incubator. You would have to purchase a brand new one and order a separate O2 control and the engineers will incorporate it for you. Hope this helps.
May
Re: ivf
Not that I think this discussion can accomplish much; but how many failed cycles are we talking about and has your clinic had success in the past?
If you\'ve been having positive pregnancy tests but no fetal hearts?
Fert rates of "up to" 80% aren't particularly high, in fact I'd suggest that's about average for ICSI with ejaculated sperm, and IVF. Good embryo quality I feel used to be based on the numbers of 8, 9, 10 cells on D3, and now on the numbers of good blastocysts on D5.
When looking at culture conditions that might have changed, look for changes in batch dates from suppliers for media AND plasticware that might have made a difference.
Simon Aiken
Re: ivf
To get good result in IVF is multifactorial. Quality and grade of embryos that you have transfered in the patients, patient's endometrium thickness (triple line) and its vascularity, ET technique, post ET patient supportive medicine, your lab quality control, Patient's age and hormonal level, in which stage you transfer your embryo, two cell, four cell, eight cell or blastocyst. Your embryo loading technique, type of ET catheter...
Dr.Pratiksha joshi IKDRC
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