IVF News

News: HFEA Consultations: Have your say! The HFEA has launched two consultations that will affect the way the regulator works with both patients and clinics. HFEA - 0 Comments.

News: HFEA welcomes Royal Assent for HFE Act Ground breaking new legislation updating the law on assisted reproduction and embryo research received Royal Assent on 13th November. HFEA - 0 Comments.

News: Study Shows It May Be Possible to Preserve Child-Bearing Ability in Young Girls with Cancer Research appearing in the on-line edition of Fertility and Sterility revealed that it may be possible for girls suffering from cancer in childhood to preserve their ability to have children later in life. ASRM - 0 Comments.

IVF Jobs

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#	Position	Closing Date	Hits
1	Lead Fertility Nurse Specialist   Addenbrooke's Hospital Cambridge, United Kingdom	18 November 2008 - Interview Date 1 December 2008	306
2	Lead Clinical Embryologist   Addenbrooke's Hospital Cambridge, United Kingdom	18 November 2008 - Interview Date 3 December 2008	706
3	Senior Embryologist Nuffield Health Glasgow Hospital Glasgow, United Kingdom	28th November 2008	29
4	Embryologist The Agora Gynaecology & Fertility Centre Brighton & Hove, United Kingdom	28th November 2008	113
5	Re-advertisement : Trainee Embryologist Band 6 Belfast Health & Social Care Trust Belfast, United Kingdom	25/11/2008	92
6	Re-advertisement : Embryologist (Pre-Registrant) Band 6 Belfast Health & Social Care Trust Belfast, United Kingdom	25/11/2008	51
7	Re-Advertisement : Embryologist (Trained) Band 7 Belfast Health & Social Care Trust Belfast, United Kingdom	25/11/2008	62
8	Fertility Nurse Isis Fertility Centre Colchester, United Kingdom	31.1.09	55
9	IVF SPECIALIST London Fertility Centre London, United Kingdom	1 December 2008	171
10	Fertility Nurse The London Bridge Fertility, Gynaecology and Genetics Centre London, United Kingdom	Until position is filled	155
11	Senior Clinical and Research Embryologist University of Dundee Dundee, United Kingdom	November 28th 2008	419
12	Embryologist The Kilkenny Clinic Kilkenny, Ireland		2401
13	Consultant Gynaecologist with Specialist Infertility Training The Kilkenny Clinic Kilkenny, Ireland		1705
14	Senior Embryologist Cork Fertility Centre College Road, Ireland		5345

Resumes

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#	Title	Country	Photo	Hits
1	nahed alhanbali tecnition in IVF&ICSI lab	Saudi Arabia	-	50
2	Sule Dogan Embryologist/ Andrologist/ research assistant	Turkey		153
	"Female and 27 years old."
3	Momen Motawy Clinical embryologist	Egypt		148
4	SHATRUGHNA PATIL EMBRYOLOGIST	India	-	137
5	tianwu qiao embryologist	Germany		171
	"qiaotianwu@163.com"
6	Nikolce Ognenovski Embryologist	Macedonia		204
7	Anna Nagar Senior Embryologist	India	-	253
	"To pursue a career which makes me deal with current scientific procedures and latest techniques in human embryology and assisted reproductive technologies. I am further interested to even work on pre implantation genetic diagnosis."
8	SAMAR PAL EMBRYOLOGIST	India	-	223
	"to work in a good environment with team members and achive the goals"
9	mahmood erfanian trainee embryologist	Iran	-	193
10	Md.Abdur Rahman Lab Technician	Bangladesh		226
	"IUI,IVF,ICSI,PCR and developing Semen Analysis."

More resumes

IVFMail

<< Add your questions here >>

#	Title	Date Added	Hits	Replies
1	Late division	17 November 2008	12	0
	We have a lady 37 years old with 2 not very good cells for ICSI. After 2.5 days one of the cell divided in 2.( with no PN in the morning) I am wondering what might be the reason for this late dividing? Thanks Bojana B. [ Reply here ]
2	Type of water	13 November 2008	23	0
	Hi there, I wanted to know if there is a difference between sterile, de-ionised and distilled water. Rana Yanis [ Reply here ]
3	RH%	13 November 2008	34	1
	What is the RH% recommended for embryo incubation in CO2 incubator units? Is 99% used and recommended? What range should be used? Rana Yanis [ Reply here ]

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Replies

Re: RH%
Incubators part three - relative humidity.

You want maximum humidity if you are doing embryo culture, even if you have oil overlays for your dishes, tubes and so forth. If you are doing open culture, you really - stress really - need to maintain maximum humidity.

First, older incubators, and a number of the new ones, rely on CO2 sensors that require maximum humidity for accuracy.

Second, anything placed into a dry incubator will be subject to dehydration at 37C - for example, open cultures (no oil overlay) will dehydrate quickly, raising osmolality to extremes and killing cells quite readily. And with oil overlay, I still worry about humidity - I\'ve seen the character of the oil change under dry, heated conditions, and that may translate to problems in the microdrops.

As for measuring humidity, why spend money on a tool to do this - 98%, 99%, etc.? The easy answer is to maintain at least one large pan of water in the incubator at all times, and if you are in a very dry environment, a second pan does not hurt.

I don\'t trust the manufacturer\'s recommendations when it comes to water pans - I have two newer incubators where the single pan provided might hold about 1 liter - so I purchased large rectangular glass pyrex baking pans that hold about 4 times the water, and use one of these in addition to the little pan supplied with the incubator.

Change the water every week to two weeks, but keep the pan or pans full. If your incubator has a door heating unit, you may not see condensation on the glass, but often you will. Condensation is a good sign that you are maximizing humidity.
Michael L. Reed Center for Reproductive Medicine of New Mexico

Re: Embryo fragmentation
I would also suggest that you take a very close look at the endotoxin load across your system, by retesting of products, media, and in particular, re-evaluation of the protein used in the culture, no matter what the certificates of analysis state.
Michael L. Reed Center for Reproductive Medicine of New Mexico

Re: Halosperm
Firstly, We used this test last year. my master thesis is about sperm DNA fragmentation. but I used this test diffirent research.We used to stain STAT III stain which contain Wrigt\'s Giemsa stain solution. It\'s work.detected by bright microscopy for counting halos.and was detected by according to literature.

Grade 1 : big halo (no fragmentation)
Grade 2 : medium halo (no fragmentation)
Grade 3 : small halo (fragmentation this step is complicated that is needed by experience)
Grade 4 : no halo (fragmentation)
Grade 5 : no halo and no stain or other (fragmentation)

I hope to help for your question
Shule

Re: Gas Concentrations in Trigas Incubators used in IVF
As regards the triple gas mixtures we are totally at the mercy of suppliers. It on their assurance that we have to depend on. In a certain brand of incubators which strictly demand the triple gas mixtures, it would be like gambling with our IVF cycles. Though at times luck favours the brave but in two very prestigious labs in India, the results came crashing down thanks to the triple gas mixtures. Instead of us jumping into the mol bio and physico chemical jargon, we should nip the problem in the bud, else our 5 or 6% co2 are ideal.
kersi m avari free lance embryologist