IVF News

News: MPs back creation of human-animal embryos British scientists will be allowed to research devastating diseases such as Alzheimer's and Parkinson's using human-animal embryos, after the House of Commons rejected a ban yesterday. An amendment to the Human Fertilisation and Embryology Bill that would have outlawed the creation of "human admixed embryos" for medical research was defeated in a free vote by a majority of 160, preserving what Gordon Brown regarded as a central element of the legislation. [ Visit Website ] Times Online - 0 Comments.

News: New technique to identify viable IVF embryos A group of Australian scientists has used a new genetic analysis technique to assess IVF embryos, to identify those most likely to develop in the womb. The findings were published in the journal Human Reproduction last week. Charlotte Maden - 0 Comments.

News: US Supreme Court refuses to hear embryo appeal The US Supreme Court has declined to hear an appeal from a Texan woman after state courts refused to grant her permission to implant embryos created using the sperm of her ex-husband, after he withdrew his consent. Antony Blackburn-Starza - 0 Comments.

News: Researchers create genetically engineered human embryo A research team at Cornell University in New York has created what is believed to be the first genetically engineered human embryo Rachael Dobson - 0 Comments.

IVF Jobs

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#	Position	Closing Date	Hits
1	Embryologist [ Featured ] London Female and Male Fertility Centre LONDON, United Kingdom	08. 06. 2008	1251
2	Trainee Embryologist (Full Time) CARE Fertility Manchester, United Kingdom	9th June 2008	151
3	Embryologist (Full Time) CARE Fertility Manchester, United Kingdom	9th June 2008	106
4	Infertility Specialist The London Bridge Fertility, Gynaecology and Genetics Centre London, United Kingdom	15th June 2008	127
5	Senior Clinical Embryologist BMI The Chaucer Hospital Canterbury, United Kingdom	30 June 2008	116
6	Junior Embryologist (Pre registration)/Andrologist Homerton Hospital London, UK	Closing date: 29 May 2008.	201
7	Embryologist Southmead Hospital Bristol, United Kingdom	12 June 2008	144
8	Senior Embryologist Embryology New York, United States		310
9	Lecturer in Clinical Embryology Leeds Institute of Genetics, Health and Therapeutics Leeds, United Kingdom	17 June 2008	195
10	Embroyologist Belfast Health & Social Care Trust Belfast, United Kingdom	6th June 2008	201
11	Embryologist Acacio Fertility Center Laguna Niguel, United States		323
12	Clinical Research Fellow Midland Fertility Services Court Parade, Aldridge, United Kingdom	16 May 2008	174
13	Clinical Embryologist King's College Hospital ACU London, United Kingdom	6th June 2008	284
14	Consultant Gynaecologist with Fertility Training The Kilkenny Clinic Kilkenny, Ireland		234
15	Research Nurse (Gynae Ultrasonography) - (One full-time post or two part-time posts) Human Resources Department Nottingham, United Kingdom	23 May 2008	202
16	Senior Clinical Embryologist Assisted Conception Unit Edgbaston, United Kingdom	Monday 2nd June 2008	399
17	LABORATORY MANAGER South East Fertility Clinic Tunbridge Wells, United Kingdom	1st June 2008	380
18	Embriologist Sergey Yakovenko Moscow, Russian Federation		672
19	Embryologist The Kilkenny Clinic Kilkenny, Ireland		582
20	full time embryologist McGill Reproductive Centre montreal, Canada	30 june 08	1173
21	Consultant Gynaecologist with Specialist Infertility Training The Kilkenny Clinic Kilkenny, Ireland		671

Resumes

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#	Title	Country	Photo	Hits
1	Ahmad Al-zyoud endrology	Jordan	-	47
2	Amol Apte research officer	India	-	248
3	Yee Siew Yin Laboratory Manager or Senior Embryologist	Malaysia		384
	A well trained & fully qualified embryologist with 7 ½ years of experience, I am seeking a position to join a dynamic & progressive organization where I can expand and fully utilize my abilities towards the benefit and development of the organization. I believe that I possess all the necessary & transferable skills to be successful in such a post.
4	John scientist and embryologist	Kuwait	-	345
5	Pravin Shinde Embryologist	India	-	243
	Cofirmed experience in the field of ART with good sucess rates and to see that each ooctye becomes embryo, each embryo becomes blastocyst and each blastocyst implants and becomes a baby as a result.
6	Lucy Richardson, Ph.D Trainee Embryologist/Embryologist	United Kingdom		595
	I am an enthusiastic individual looking to join a dynamic team of embryologists where I can apply my scientific abilities to a clinical setting.
7	Migblinkpo Messan DOSSOU Physician in Reproductive Center	Togo	-	194
	To work in Reproductive Center over the world to achieve my dream as specialised physician in reproductive medicine.Since i got my degree i don't get any center to work in and i still ordinary physician.
8	Momen motawy Embryologist	Egypt		340
9	Sonali Pandya Trainee Embryologist	United Kingdom	-	311
	I am an enthusiastic MSc graduate looking to join a dynamic team of embryologists where I can apply my scientific abilities to clinical applications. I am dedicated in everything that I apply myself to and look forward to the rewarding challenges that a career in clinical embryology will bring.
10	Chengjun Liu Embryologist	China		287
	Embryologist with technological work and research., it was better if have a chance for a doctor degree

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Re: Overnight IVF insemination concentration
A good question, and the answer in terms of sperm concentration per egg per ml really depends on the system you are using for insemination - well dishes vs test tubes vs micro or macrodrops. You will see a lot of references to sperm per egg per ml, designed originally for use with well dishes or test tubes. For example, 50,000 motile sperm per egg per ml was/is common with well dishes, each dish having one ml medium either under oil or with lots of medium around the well in a moat, and a single egg in the one ml volume. Similar for test tubes, where an egg is placed with one ml medium. The dynamics of the well, or tube are different as well, as wells may have a greater diameter than the test tube, changing the local concentration around the egg, and hence you may not need quite as many sperm per egg per ml in a narrow test tube. In micro or macrodrops, the concentration/egg/ml may be the same, but you will use a lot less sperm. For example, I target a final drop size of 200 microliters after the addition of sperm, so to get to 50,000 sperm/egg/ml, I'm adding approximately 10,000 sperm to the drop. I'll increase the number of sperm per drop if I add an extra egg (up to three eggs per drop), and the maximum number of sperm per drop that i use is approximatley 20,000 to 25,000 sperm. You can use fewer sperm in micro or macrodrops due to the fact that the drops trap the sperm in closer approximation to the egg(s). I believe that I've seen a concentration as low as 2,000 sperm per egg in a microdrop setting, but I would not personnaly want to go that low with sperm numbers.

If you are using micro or macrodrops, you should be even more careful of the pH management, as a lot of sperm in a confined volume will change the pH, as metabolic acids are released by the motile sperm, you'll see a shift towards a more acidic environment. This is very easy to test with medium that has phenol in it. You can inseminate drops, wells, tubes, whatever with different numbers of motile sperm, and watch what happens overnight.
Michael L. Reed Center for Reproductive Medicine of New Mexico

Re: Fragmentation
Fragmentation increases with increasing toxicity in the environment, check your air quality inside the incubators and culture room. Rinse the disposables with media before use. I wish you all the best Alok
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