IVF News

News: IVF News Update 'Capsule' could cut costs and time of IVF, Scientists argue for freedom to develop sperm and eggs from stem cells, Outgoing Italian health minister revises IVF guidelines, 'No early menopause' through IVF. IVFPODCASTS.COM - 0 Comments.

Announcement: Hudson Valley Fertility opens State of the Art IVF and Fertility Center Hudson Valley Fertility has opened a state of the art IVF and Fertility Center at 841 Route 52 in Fishkill, in the beautiful Hudson Valley. The Center offers cutting edge technology combined with sensitivity and compassion. The practice also incoporates acupuncture, stress reduction and other complementary strategies as part of a complete wellness approach to the treatment of infertilty and other reproductive issues. Daniel Levine, MD - 0 Comments.

News: IVF does not lead to early menopause A group of researchers at the Bourn Hall Clinic, Queensland University of Technology and the Weill Medical College of Cornell University, New York, have concluded that IVF treatment does not hasten the onset of the menopause or the severity of symptoms, having investigated the first generation of IVF patients. Katy Sinclair - 0 Comments.

IVF Jobs

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#	Position	Closing Date	Hits
1	Embryologist [ Featured ] London Female and Male Fertility Centre LONDON, United Kingdom	08. 06. 2008	913
2	Consultant Gynaecologist with Fertility Training The Kilkenny Clinic Kilkenny, Ireland		94
3	Senior Embryologist/ laboratory manager Fertility Unit Southampton, United Kingdom	Friday 16th May 2008	280
4	Research Nurse (Gynae Ultrasonography) - (One full-time post or two part-time posts) Human Resources Department Nottingham, United Kingdom	23 May 2008	112
5	Senior Clinical Embryologist Assisted Conception Unit Edgbaston, United Kingdom	Monday 2nd June 2008	237
6	IVF lab clinical embryologist Dr Erfanian IVF Clinic mashhad, Iran		270
7	CLINICAL EMBRYOLOGIST St. Mary's Hospital Manchester, United Kingdom	Closing date: 22nd May 2008.	233
8	LABORATORY MANAGER South East Fertility Clinic Tunbridge Wells, United Kingdom	1st June 2008	228
9	Clinical Research Fellow Midland Fertility Services Court Parade, Aldridge, United Kingdom	16 May 2008	222
10	short term locum The Agora Gynae and Fertility Unit Hove, United Kingdom	June	434
11	Embriologist Sergey Yakovenko Moscow, Russian Federation		526
12	Embryologist The Kilkenny Clinic Kilkenny, Ireland		478
13	full time embryologist McGill Reproductive Centre montreal, Canada	30 june 08	983
14	Consultant Gynaecologist with Specialist Infertility Training The Kilkenny Clinic Kilkenny, Ireland		586

Resumes

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#	Title	Country	Photo	Hits
1	Amol Apte research officer	India	-	150
2	Yee Siew Yin Laboratory Manager or Senior Embryologist	Malaysia		240
	A well trained & fully qualified embryologist with 7 ½ years of experience, I am seeking a position to join a dynamic & progressive organization where I can expand and fully utilize my abilities towards the benefit and development of the organization. I believe that I possess all the necessary & transferable skills to be successful in such a post.
3	Dejan Ljiljak Embriologyst	Croatia	-	197
4	John scientist and embryologist	Kuwait	-	279
5	Pravin Shinde Embryologist	India	-	186
	Cofirmed experience in the field of ART with good sucess rates and to see that each ooctye becomes embryo, each embryo becomes blastocyst and each blastocyst implants and becomes a baby as a result.
6	Lucy Richardson, Ph.D Trainee Embryologist/Embryologist	United Kingdom		477
	I am an enthusiastic individual looking to join a dynamic team of embryologists where I can apply my scientific abilities to a clinical setting.
7	Migblinkpo Messan DOSSOU Physician in Reproductive Center	Togo	-	163
	To work in Reproductive Center over the world to achieve my dream as specialised physician in reproductive medicine.Since i got my degree i don't get any center to work in and i still ordinary physician.
8	Momen motawy Embryologist	Egypt		274
9	Sonali Pandya Trainee Embryologist	United Kingdom	-	254
	I am an enthusiastic MSc graduate looking to join a dynamic team of embryologists where I can apply my scientific abilities to clinical applications. I am dedicated in everything that I apply myself to and look forward to the rewarding challenges that a career in clinical embryology will bring.
10	Chengjun Liu Embryologist	China		243
	Embryologist with technological work and research., it was better if have a chance for a doctor degree

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IVFMail

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#	Title	Date Added	Hits	Replies
1	Overnight IVF insemination concentration	05 May 2008	62	1
	I would like to ask "What is the maximum concentration of sperms added in the overnight IVF culture?" 0.1 million motile sperms or above? Wendy [ Reply here ]
2	2 polar bodies in oocyte before ICSI?	05 May 2008	67	1
	When the oocyte appears one polar body/fragmented polar body, that is MII so that we can do ICSI for it. But if I observe 2 distinct polar bodies (likely) in oocyte, should I do ICSI for it? Will it be a normal embryo too? Wendy [ Reply here ]

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Replies

Re: multinucleated blastomeres
I have done FISH(PGD) before on the multinucleated blastomeres on day 3, they are all fragmented without any normal signal confirmed. So most probably, they are all abnormal. But in your case, you said that you saw a single nucleus/blastomere on day 2 while multinucleated on day 1. Do you mean that the multinucleated is 2? If so, maybe the blastomere haven\'t ready to divide on day 1, so that you see single when they divided into 4 cells, just purpose not sure (although it should be divided before forming nucleus). But in the early stage, mosaicism should be considered. So I prefer to freeze it for FET cycle and select the good embryo for fresh ET.
Wendy Man

Re: Fragmentation
Large amounts of fragmentation are usually due to contaminants, especially endotoxin. I would check to make sure the medium never gets above 37 C. I would never set a dry block to 39 to get it to rad near 37 as at the very bottom where the egg sits, it might be 40 C. Next, I would make sure that you have done appropriate QC bioassays for all contact materials. I would suspect syringes, dishes, plates, etc.
Kimball O Pomeroy ARMS

Re: Fragmentation
You have to check your sperm preparation. Do a second swim up and get the clean motile sperms. It seems you are doing only IVF and not ICSI. If that is the case, also check the water in the humidity chamber of your CO2 incubator. You must change the water every 10 days and the level must be maintained or else fragmentation comes. You should also know the pathology of the patient and if she is an untreated PCOS or Endometriosis, you are bound to get fertilization but embryos with fragmentation. Why don\'t you divide the oocytes, half by IVF and half by ICSI. You yourself can find the reason.
Dr.Ravindranath Visiting Senior Embryologist

Re: Fragmentation
Try culturing your embryos in microdrps with 3-5 embryos per drop under oil. Also to eliminate variation in pH and control VOCs culture a dessicator filled with a medical grade trigas mixture of 6% co2,5%o2 and balence nitrogen. pH chnges and increased VOC levels are usually responsible for fragmentation in the lab. I would next examine the stimulation protocols and do a comparitive study to see if fragmentions is occuring across the board or is seen in cases with high e2 levels before HCG.Hope you find some answers.
Lakshmi Sharma RCARC

Re: Fragmentation
PH is the magic kye on embryo quality. strive to reach a PH between 7.22 and 7.25 and your problem will be solved, as much there is no medium contamination. Hope this help
Orestes Prudencio

Re: Piping for CO2 to incubators
Copper works very well in IVF setting for CO2 delivery. Copper may be used in incubators for germicidal reasons but copper isn\'t volatile. It will not change state from a solid to a gas.
Antonia Gilligan Alpha Environmental, Inc

Re: QC
Have you considered an actual analysis of the carbon dioxide? A US EPA TO-15 VOC and TO-11 aldehydes will provide real information on the purity of the CO2. Also keep in mind that as the phase change occurs the composition of impurities will increase significantly
Antonia Gilligan Alpha Environmental, Inc

Re: Embryos arrested and lysed on day 3 and 4
High levels of aldehydes can cause this problem we have seen it three IVF facilities in North America
Antonia Gilligan Alpha Environmental, Inc

Re: Overnight IVF insemination concentration
I usually add approximatly 100,000 sperm per oocyte.
Sevinc Ege JinePOL

Re: thin zp
We have had a patient with very thin large zonas. They also had a large perivitiline space. We were very careful when denuding oocytes and if necessary left some cummulus cells in place. We found it also necessary to culture the embryos separatly as they can become stuck together. There are number of papers about zona free embryos.
Erin Shummly

Re: oocyte quality
Please check the patient\'s pathology whether she has Endometriosis or untreated PCOS. There is always asynchrony in the oocyte growth and the cohort shall carry similar quality, i.e the embryo quality suffers and implantation failure shall be there if there are oocytes of different quality. Of course along with her age, check her basal FSH , LH, Testosterone, insulin, may be you may catch the culprit hormone. Best of luck
Dr.Ravindranath Visiting Senior Embryologist

Re: Oocyte quality
Most of the time this event is related to one bad gonadotropin batch, provided you are sure of your lab. Can you tell us which company gonadotropin you were using with batch number.
Deepak Goenka IHR