IVF News

New Book: Human Preimplantation Embryo Selection [Kay Elder, Jacques Cohen] A comprehensive overview to guide the ART practitioner in choice of techniques and evaluation of benefits and drawbacks. In summary, application of increasingly advanced new technology has allowed the field of embryo evaluation to evolve rapidly and dramatically over the past five years, and this book will provide an up-to-date overview and reference source. [ Visit Website ] IVF Books - 0 Comments.

New Book: In Vitro Fertilization: A Practical Approach [David K. Gardner] Supplying a comprehensive and hands-on approach to in vitro fertilization (IVF), this source presents established state-of-the-art procedures and techniques, as well as the most current research in the field. Expert contributors explore the history of IVF and progress to future research pathways. Additional coverage includes preimplantation genetic diagnosis, oocyte retrieval, oocyte donation, and micromanipulation procedures such as ICSI, assisted hatching, and embryo biopsy. [ Visit Website ] IVF Books

IVF Jobs

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#	Position	Closing Date	Hits
1	Embryologist [ Featured ] Center for Women's Reproductive Care New York, United States	Open	904
2	Locum Senior Embryologist/Laboratory Manager Obstetrics and Gynaecology Southampton, United Kingdom	26 February 2008	124
3	Trainee Clinical Embryologist The Hull IVF Unit Hull, United Kingdom	March 7th, 2008	456
4	Consultant Gynaecologist with Specialist Infertility Training The Kilkenny Clinic Kilkenny, Ireland		219
5	Clinical Embryologist Eugonia Athens, Greece	7 March 2008	396
6	Experienced Clinical Embryologist The Bridge Centre London, United Kingdom	19th February 2008	307
7	Embryologists Bristol Centre for Reproductive Medicine Bristol, United Kingdom	Closing date: 14 February 2008	488
8	Senior Registered Nurse - full time. Wessex Fertility southampton, United Kingdom	15 February 2008	232
9	Embryologist St George's House London, United Kingdom	31st January 2008	835
10	Clinical Molecular Biologist The Lister Fertility Clinic London, United Kingdom	29.02.2008	501
11	Senior Embryologist The Lister Fertility Clinic London, United Kingdom	29/02/2008	659
12	Senior Staff Nurse - IVF The Lister Fertility Clinic London, United Kingdom	31/01/2008	478

Resumes

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#	Title	Country	Photo	Hits
1	Hossam Elmahdi Embryologist	Egypt		71
	Objectives: As a clinical embryologist looking for a challenging position with your center where I would be to apply my skills in the areas of IVF technologies. I established and maintained intensive operation of an embryological IVF Lab during more 5years.
2	Yassin Ellawi Embryologist	Syrian Arab Republic	-	87
3	ANA FRANCES EMBRIOLOGYST	Spain	-	145
	I am looking for a job like an embriologyst or researcher in molocular biology
4	Pravin Shinde Consultant Embryologist	India	-	122
	To Do the quality work and help the Infertile couples to enjoy the greatness of Parenthood.
5	Hongyu Qiu Embryologist / clinical researcher	United States	-	203
6	Ron Berry Embryologist	United States	-	387
7	Natalia Danilova embryologist, Senior Embryologist, S.R.	Russian Federation	-	307
	Objectives: As a clinical embryologist I am looking for a challenging position with your company where I would be to apply my skills in the areas of IVF technologies. I established and maintained intensive operation of an embryological IVF Lab during more 3,5years.
8	Balasaraswathi Krishna Junior Embriyologist	India	-	307
	To become a successful professional in the field of Biotechnology and to work in an innovative and competitive world. Learning and Training from the job should be more so that I can develop myself.
9	DR BEENA MUKTESH Consultant IRegular/visiting nfertility and IVF	India	-	326
	To serve as Visiting / Regular consultant (Infertility ,IVF) at the prestigeous hospital or setup and to deliver the best of my skill and knowledge
10	Peter Freelance clinical embryologist and stem cell consultant	United Kingdom	-	391
	To provide consultancy worldwide on all aspects of clinical embryology (including locum work) and stem cell biology including trouble shooting, all types of stem cells including adult, business development, laboratory/service development and scientific advisory board membership.

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IVFMail

<< Add your questions here >>

#	Title	Date Added	Hits	Replies
1	sterilisation of embryo biopsy needles	22 February 2008	14	0
	I have been told that ideally embryo biopsy needles need to be washed in deionised water followed by an acid-wash before sonication. Does anyone know what should be used for the acid-wash? Dilute bleach or related substance or does a particular acid have to be used? Thank you c carolyn Cummins [ Reply here ]
2	Ozon	22 February 2008	11	0
	Greetings! After upgrading our lab new powerful ultra-violet lamps were mounted. Room for manipulations is small. Therefore air in lab is ionized and it is smelt some ozon. Does anybody know how ozon can influence on gametes and embryos? Naturally, ionized air everywhere and in incubators too! Serj [ Reply here ]
3	morphology	18 February 2008	43	1
	does it need to stain spematozoa in order to find morphology? maryam [ Reply here ]

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Replies

Re: Morphology
Regarding staining of sperm for morphology, there are certainly details of live sperm that can be seen with conventional microscopy that do not require stains. Fixed, nonstained sperm can be visualized to some degree with phase or brightfield microscopy, and by DIC, however the fixation process iteself will change the shape of the sperm to some extent. And there are excellent papers by Dr. Bartoov regarding high magnification analysis of living sperm for selection for ICSI.

Staining gives you access to fine details on larger numbers of cells (manual or computer analysis) without requiring complex imaging systems. In addition, the cells can be archived, while living sperm obviously cannot be archived. There are a number of stains available commercially that yield high repeatability between specimens, and are easy to use so that you can achieve good repeatability between technicians.

From a regulatory standpoint, there are also larger peer groups available for comparison of data when using stained preparations.

But the important question: what is your clinical need or application? In other words, how would you use the sperm morphology data? If you look at the proficiency testing data from the American Association of Bioanalysts you will find multiple categories for interpretation, and within each category multiple stain types. If you desire to use the \"strict\" criteria method, the choice of staining method is very important. For any method to be meaningful, at the least you will need to look at your data against your own patient population - but it is very helpful to use a method where data is available and comparable either by proficiency testing or by comparison of your correlates to publications by others.

A good starting point would be to attend a seminar on sperm morphology, or to obtain either a more recent WHO manual, or the 2002 ESHRE manual (which I believe used to be avialable from the Human Reproduction free article website).

Mike
Michael L. Reed Center for Reproductive Medicine of New Mexico